Figure 5.

Heatmap visualization of protein expression profiles of different sample preparation strategies. A dendrogram of three extraction methods with three technical replicates was shown at the top. Protein expression values were z-score normalized prior to clustering. Hierarchical clustering of protein expression profiles identified four groups. Gene ontology enrichment analysis was performed for each protein group and cellular components of proteins, which had higher extraction efficiency in the SCAD and FASP methods compared with the urea method (right, upper) or higher expressions in the urea and SCAD methods versus FASP (right, lower).