(A) The list of 295 entries annotated as testis-expressed in the Eurexpress database were compared to 491 Sertoli cell expressed genes from a microarray of sorted cells at 13.5 dpc (Jameson et. al., 2012). 35 genes were represented in both sources (see Supp Table 2). (B) Out of these 35 genes, 5 known Sertoli cell marker genes were identified: Amh, Col9a3, Etv5, Ptgds, and Tyro3. 16 novel Sertoli cell candidate genes with a quality ranking of 1 or 2 were annotated in the Eurexppress database: Adamts16, Atp8b2, Ctnna2, Cxadr, Dsp, Gjb2. Gstm7, Lsr, Mpr1, Pgepep1, Slc20a1, Slc38a1, Tesc, Tmcc3, Tpd52a and Trank1. The testis region is demarcated by a dashed line. a: adrenal; k: kidney; m: mesonephros. Scale bar: 0.5 mm.