Fig. 5.

THC deters cell migration and abolishes serum-induced actin reorganization/focal adhesion assembly. a Cells were treated with vehicle, THC or PP1 for 24 h followed by the analysis of cell migration using Transwells. Data are means ± SD. n = 4. *, p < 0.01 vs control (0). **, p < 0.005 vs control (0). b Cells were grown to confluency followed by treatment of vehicle, THC or PP1 for 24 h. A scratch was made with a fine pipette tip and cells were kept in medium containing 1% FCS with or without THC or PP1. Images were taken at 0 and 24 h under a phase contrast microscope. c. H1299 cells were treated with vehicle or 10 μM THC for 24 h in serum-free condition, then stimulated with 10% FCS for 1 h followed by immunostaining with phalloidin (red) and paxillin mAb (green). A representative view was chosen for photography