Fig. 6.

ISL and THC suppress in vitro invasion and in vivo tumor development. a H1299 cells were treated with vehicle, 10 μM THC, ISL or PP1 followed by Matrigel invasion assay. Cells on undersurface of invasion chambers were stained and images were taken under a phase contrast microscope. b Quantitation of invasive cells (on undersurface). Data are the mean ± SE. n = 3. *, p < 0.01 vs vehicle. c Luciferase-containing H1299 cells were injected into nude mice for 2 weeks followed by administration of 25 mg/Kg ISL or THC. Tumor development was monitored by measuring fluorescence intensity once every 3 days starting from 1 week after tumor cell injection. Data are the mean ± SE. n = 8. d Tumors were imaged in Xenogen system 3 weeks after treatment. e Tumors were excised at the end of treatment and weighed. ##, p < 0.05 vs vehicle