Figure 4.

Diabetic NKO mice display lower levels of renal inflammatory cytokines. Kidneys from diabetic WT, NKO, and CKO mice and their respective nondiabetic (Non-D) controls were collected after 6 months of diabetes. Whole-kidney homogenates were assessed for (A) IL-1β, (B) TNF-α, and (C) IL-6 by ELISA. Results were expressed as picograms of cytokine per milligram of total kidney protein. n=5–8 per group. *P<0.05; **P<0.01. Data are represented as individual values for each mouse (dots). Horizontal bars represent the mean±SEM. Immunolocalization of renal (D) IL-1β, (E) TNF-α, and (F) IL-6 was performed by immunohistochemistry; n=5. Twenty fields per sample were analyzed. Original magnification, ×40. Immunohistochemistry quantification in Supplemental Figure 3.