Figure 8.

Inhibition of AcSDKP synthesis increases inflammation and microalbuminuria in diabetic NKO mice. Whole-kidney homogenates were assessed for (A) IL-1β and (B) TNF-α by ELISA. Data are expressed as picograms of cytokine per milligram of total kidney protein. (C) Microalbuminuria was measured by ELISA and expressed as micrograms of albumin per milligram of creatinine. (D) Urinary albumin from (C) was plotted against urinary AcSDKP from Figure 7A, combining WT and NKO diabetic mice receiving either vehicle or S17092. Plots indicate a negative correlation between albumin and AcSDKP in the urine. Urinary (E) prostasin and (F) furin were assessed by western blot in urine samples concentrated five-fold for nondiabetic and 20-fold for diabetic animals using centrifugal filters with a molecular mass cut-off of 10 kD. Immunoblots were performed with an amount of concentrated urine equivalent to 10 µg of creatinine. Diabetic WT group was considered as 1. n=5–7 per group. *P<0.05; ***P<0.001. Data are represented as individual values for each mouse (dots). Horizontal bars represent the mean±SEM.