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. 2018 Oct 3;19:724. doi: 10.1186/s12864-018-5099-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Common measures of the species rank exhibit widely different correlations with gene content similarity in Streptomycetaceae. The 16S rRNA sequence showed little correlation with gene content similarity (R² = 0.38), whereas the rpoB gene sequence displayed good correlation (R² = 0.75). Two genome-wide measures of similarity, average nucleotide identity (ANI) and alignment fraction (AF), were strongly correlated with gene content similarity. Horizontal dashed lines denote commonly used species-level cutoffs for each measure (e.g., > 97% 16S sequence identity)