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. 2018 Oct 3;18:945. doi: 10.1186/s12885-018-4853-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Phenotypic characterisation of isolated cells from colorectal cancer samples. Percentage of surface marker expression in cells from tumour (T, grey) vs. peritumour (PT, white) areas, immediately after isolation from patients with CRC (n = 15), as determined by FACS (a). Morphological appearance (10×) of tumour (b) and peritumour cells (c) immediately after isolation procedure. Percentage of PD-L1⁺ cells within tumour (T, grey boxes) and peritumour (PT, white boxes) areas, as measured by FACS (d). Percentage of PD-L1⁺ cells on PanK⁺ and EpCAM⁺ gated tumour cells from D (e). * p < .05, ** p < .01 using a Wilcoxon test