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. 2018 Oct 3;18:945. doi: 10.1186/s12885-018-4853-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Characterisation of infiltrated T-lymphocytes in tumour and peritumour areas in colorectal cancer samples. Percentage of CD3⁺ cells within tumour (T, grey boxes) and peritumour (PT, white boxes) areas from patients with CRC (n = 15), as analysed by FACS (a). Percentage of CD8⁺ cells (b) and CD4⁺ cells (c) on CD3⁺ gated populations in A. Expression of PD-1 on CD4⁺ gated populations in C (d). * p < .05, ** p < .01 using a Wilcoxon test