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Production and purification of the recombinant abundant Leishmania proteins. The eight abundant Leishmania protein genes were PCR amplified from L. donovani and cloned into the pET29w expression vector. Recombinant proteins containing an N-terminal His-tag were expressed in E. coli BL21 and purified with Ni-NTA agarose. Purity was evaluated by SDS-PAGE and Coomassie blue staining. These recombinant Leishmania proteins were sent out for rabbit polyclonal antibody production
