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Leishmania 40S ribosomal protein S12 sandwich ELISA displayed high sensitivity and specificity. This ELISA assay can detect as low as 1 pg of purified recombinant 40S ribosomal protein S12 (a), the lysate of 60 Leishmania donovani parasites alone or mixed with 500,000 PBMCs prepared from healthy controls (b), which equals about 60 Leishmania amastigotes in 750 μl blood. Note: the detecting signal intensities were slightly lower in spiked PBMC lysate due to the matrix effect
