Figure 5.
ACSL-4 induced ferroptosis. (a) Four different CRCs, CacO2, NCI-H508, HCT-116, and DLD-1, were cultured with different concentrations of erastin (left panel) or 5 μM erastin with or without bromelain (right panel) and cell death was analyzed. (b) Four different CRCs, CacO2, NCI-H508, HCT-116, and DLD-1, and normal colon cells, CCD18co, were treated with or without bromelain and the expression level of ACSL-4 was analyzed. (c) DLD-1 cells were treated with erastin, siACSL-4, or bromelain. Cell death was quantified by PI staining coupled with flow cytometry (upper panel) and lipid ROS was measured (lower panel). (d) Schematic diagram describing the action of bromelain in ferroptosis. The results are shown as the mean, with error bars representing the 95% confidence interval (lower/upper limit); * p < 0.05.