Table 1. Primer information for qPCR.
| Gene | Accession number | Sequence (5’– 3’) | Size (bp) | Annealing (°C) |
|---|---|---|---|---|
| PPARα | EF534215 | F- CCTGGCTTCTCCAATCTTGAC R- GCAAATGATGGCAGCGACA |
252 | 59 |
| PPARγ | NM_181024 | F- GTGAAGCCCATTGAGGACAT R- AGCTGCACGTGTTCTGTCAC |
148 | 58 |
| LPL | NM_001075120 | F- ACTTGCCACCTCATTCCTG R- ACCCAACTCTCATACATTCCTG |
119 | 56 |
| ACOX | NM_001035289 | F- GAGTGAGCTGCCTGAGCTTC R- TTGTCCAGGACGTGAAAGC |
62 | 59 |
| CPT-1 | GW342984 | F- GTCTCCAAGGCTCCGACAA R-AAGACCCGAATGAAAGTA |
193 | 58 |
| FABP4 | BT10868 | F- GCTGCACTTCTTTCTCACCT R- TTCCTGGTAGCAAAGCCCAC |
140 | 58 |
| GAPDH | NM_001034034 | F- CACCCTCAAGATTGTCAGC R- TAAGTCCCTCCACGATGC |
98 | 57 |
PPARα, Peroxisome proliferator receptor alfa; PPARγ, Peroxisome proliferator receptor gamma; LPL, Lipoprotein lipase; ACOX, Acyl-CoA oxidase; CPT-1, Carnitine palmitoyl transferase; FABP4, Fatty-acid-binding protein4; GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.