Fig. 3. Effect of EISO, α- and β-santalol on C. elegans lifespan. Survival rate of wild-type C. elegans cultured on NGM plates (from late L4 stage) containing different pharmacological doses of (A) EISO, (B) α-santalol and (C) β-santalol under normal culture conditions. (D) Survival curve of wild-type worms treated from embryo and raised at normal culture conditions on NGM plates carrying either 0.1% DMSO (solvent control) or EISO (24 μg mL⁻¹), α-santalol (32 μM), and β-santalol (16 μM). The survival curves were plotted using Kaplan–Meier survival analysis and significance levels were estimated by long-rank test in Medcalc statistical tool. The representative Kaplan–Meier survival curves from three independent runs were presented. Statistical details of these experiments are summarized in ESI Table 5.†.