Fig. 5. Effect of EISO, α- and β-santalol on the inhibition of apoptosis induced by 6-OHDA in C. elegans (n = 30–40 worms per experiment). (A) Fluorescence micrographs depicting the 6-OHDA exposure-induced germline cell death/apoptosis in wild-type N2 C. elegans. Arrow marks indicating bright distinct circles represent germ cell corpses. (B) Bar graph depicts the quantified germ cell corpses in posterior part of gonad arm of each animal across various treatments. (C) Fluorescence micrographs illustrating the protective effects of EISO against 6-OHDA-induced apoptosis and its influences on CED-1::GFP expression in engulfing cells/phagosomal surface around the cell corpses in posterior gonadal arm of MD701 C. elegans. (D) Quantified CED-1::GFP halos on the engulfing cells and nascent phagosomes. For all the experiments, 50 μM epigallocatechin gallate (EGCG) was used as positive control. Data were pooled from biological triplicates in experimental duplicates and presented as mean ± SEM. Probability levels of p < 0.05 were considered as statistically significant. *p < 0.05, **p < 0.01, ns, not significant.