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. Author manuscript; available in PMC: 2018 Oct 4.
Published in final edited form as: Chemistry. 2017 Jan 18;23(7):1694–1701. doi: 10.1002/chem.201605287

Figure 2.

Figure 2.

Synthetic and natural DDM-838 possess equivalent ability to activate the native CD8-2 T cell clone. Compound 1 prepared herein (solution phase); according to Young and co-workers (solid phase);[3b] and isolated from M. tuberculosis H37Ra, were incubated with the human T cell clone CD8-2 and CD1a-expressing K562 myeloid cells[15] over 24 h and IFNγ release measured by ELISA. DDM-838 isolated from M. tuberculosis contained 25% of the saturated analogue DDM-840,[3a] which is less stimulatory than DDM-838. Error bars indicate standard deviation among triplicates. Data representative of three independent experiments. Controls at 0 nM DDM-838 are for experiments including T cells alone, K562 cells alone, and both cell lines.