Table 1.

Molecular drug resistance and spoligotyping results for the 29 isolates detected as resistant to RIF and/or INH with AID TB Resistance INH/RIF line probe assay

AID TB resistance											TB-RINT			Spoligotyping
RIF	INH	rpoB	katG	inhA	FLQ	EMB	SLID	gyrA	embB 306	rrs	rpoB	katG 315	inhA	Lineage/SIT
R	R	531 mut	mut	wt	S	S	S	wt	wt	wt	531TTG	315C	wt	L4.6.2/Cameroon/SIT852
R	R	531 mut	mut	wt	S	S	S	wt	wt	wt	NP	NP	NP	NP
R	R	526 mut	wt Ø	wt	S	R	S	wt	ATC	wt	NR	NR	NR	L4.6.2/Cameroon/SIT852
R	R	526 mut	mut	wt	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT838
R	R	516 mut	mut	wt	S	S	S	wt	wt	wt	516GTC	315C	wt	L4.6.2/Cameroon/SIT61
R	S	531 mut	wt	wt	R/S	R	S	A90A/V, D94D/V	mut	wt	NR	NR	NR	L5/afri/WA1/orphan
R	S	531 mut	wt	wt	R	R	S	A90V	ATC	wt	NP	NP	NP	NP
R	S	531 mut	wt	wt	S	S	S	wt	wt	wt	531TTG	wt	wt	L4.6.2/Cameroon/SIT61
R	S	531 mut	wt	wt	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT61
R	S	526 mut	wt	wt	S	S	S	wt	wt	wt	526GAC	wt	wt	L2/Beijing/SIT269
R	S	526 mut	wt	wt	S	S	S	wt	wt	wt	526GAC	wt	wt	L2/Beijing/SIT269
R	S	526 mut	wt	wt	S	S	S	wt	wt	wt	NR	NR	NR	L4/T1/orphan
R	S	526 mut	wt	wt	S	S	S	wt	wt	wt	NP	NP	NP	NP
R	S	526 mut	wt	wt	S	S	S	wt	wt	wt	NP	NP	NP	NP
S	R	wt	mut	mut	S	S	S	wt	wt	wt	wt	315C	(−15)T	L4.6.2/Cameroon/SIT852
S	R	wt	mut	wt	S	S	S	wt	wt	wt	wt	315C	wt	L4.6.2/Cameroon/SIT61
S	R	wt	mut	wt	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	S	S	wt	wt	wt	wt	wt	(−15)T	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	S	S	wt	wt	wt	wt	wt	(−15)T	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	S	S	wt	wt	wt	wt	wt	(−15)T	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	S	S	wt	wt	wt	wt	wt	(−15)T	L4/T1/SIT53
S	R	wt	wt	mut	S	S	S	wt	wt	wt	NR	wt	(−15)T	L4/T1/SIT53
S	R	wt	wt	mut	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT61
S	R	wt	wt	mut	S	NR	S	wt	NR	wt	NP	NP	NP	NP
S	R	wt	wt	mut	S	S	NR	wt	wt	NR	wt	wt	(−15)T	L4/new
S	R/S	wt	wt	wt/mut	S	S	S	wt	wt	wt	NR	NR	NR	L4.6.2/Cameroon/SIT61
S	R	wt	wt	wt Ø	S	S	S	wt	wt	wt	NR	NR	NR	L4/T2/SIT316
S	R	wt	wt	wt Ø	S	S	S	wt	wt	wt	NR	NR	NR	NR

Abbreviations: AID, Autoimmun Diagnostika; TB, tuberculosis; TB-RINT, tuberculosis rifampicin isoniazid typing, the microbead-based test for detecting drug resistance; RIF, rifampicin; INH, isoniazid; FLQ, fluoroquinolones; EMB, ethambutol; SLID, second-line injectable drugs (kanamycin, amikacin, capreomycin); SIT, spoligotyping international type; R, resistant; S, susceptible; R/S, heteroresistant; mut, mutation; wt, wild-type; wt Ø, absence of hybridization on the wild-type probe; NP, not performed; NR, no result obtained; afri, Mycobacterium africanum; WA1, West African 1; orphan, pattern described in SITVITWEB but without SIT number assigned.