Skip to main content
. Author manuscript; available in PMC: 2018 Oct 4.
Published in final edited form as: Adv Biosyst. 2017 Apr 24;1(5):1700048. doi: 10.1002/adbi.201700048

Figure 2.

Figure 2

Cell patterning via ParaStamp. a) Schematic showing the cell-patterning procedure. b) Cross-sectional view of the stamped paramembrane onto a cover glass. Scale bar, 50 µm. c–e) Characteristics of cell patterning by the ParaStamp, including patterning of single and multiple MCF7 cells in two circular shapes, numbers of “9” and “0”, and symbols of “+” and “n”. Cells were stained with calcein AM. Scale bars, 50 µm in c (left panel by 4× objective lens and right panel by 10× objective lens), 100 µm in d (by 10× objective lens), 200 µm in e (by 10× objective lens). f) Comparison of two F-actin expressions in MCF7 cells cultured on glasses, with and without paramembrane patterning. F-actin and nuclei were stained with phalloidin (red) and Hoechst (blue), respectively. a.u., arbitrary unit. Data represents the mean ± SD from n = 40 (with pattern) and n = 82 (without pattern), respectively (***p < 0.001). Scale bar, 10 µm (by 63× objective lens).