Figure 8. Differential RNA expression in strains expressing mutant U3.

Volcano plot showing differential RNA expression in strains expressing mutant U3 compared to the wild type following growth in glucose medium for 36 h. RNA sequencing was performed following Ribominus selection. X axis shows log ₂ of normalized RNA fold change with negative values signifying decreased in RNA expression in the mutant. Y axis shows –log ₁₀ of the p-value. Significantly differentially expressed genes are either black (non-CLASH targets) or red (CLASH targets). ( A) U3 targets are highlighted in red; ( B) All snoRNA targets are highlighted in red.