Figure 1. Metabolites and pathways covered in the presented LC-MS/MS methods.

Metabolites from the indicated pathways covering central carbon metabolism and their branches could be measured with the use of different gradients, and a derivatization method (discussed in the main text), using a single HPLC column. Standards were used for developing multiple reaction monitoring (MRM) parameters. All abbreviations used and MRM parameters are listed in Table 1.