Table 2. LC-MS/MS parameters used for 13C and 15N labeling experiments.
| Metabolite | Q1/Q3 (Parent/
Product) |
Comments |
|---|---|---|
| 15N labeling experiment | ||
| AMP | 348/136 | Q3 has all nitrogen atoms. |
| 15N_AMP_1 | 349/137 | |
| 15N_AMP_2 | 350/138 | |
| 15N_AMP_3 | 351/139 | |
| 15N_AMP_4 | 352/140 | |
| 15N_AMP_5 | 353/141 | |
| 13C labeling experiment | ||
| AMP | 348/136 | Q3 has 5 carbons coming from the
nitrogen base. The rest of the carbons form the ribose ring. |
| 13C_AMP_1 | 349/137 | |
| 13C_AMP_2 | 350/138 | |
| 13C_AMP_3 | 351/139 | |
| 13C_AMP_4 | 352/140 | |
| 13C_AMP_5 | 353/141 | |
|
13C_AMP_5_with
labeled ribose |
353/136 | |
| 13C_AMP_6 | 354/137 | |
| 13C_AMP_7 | 355/138 | |
| 13C_AMP_8 | 356/139 | |
| 13C_AMP_9 | 357/140 | |
| 13C_AMP_10 | 358/141 | |