Fig. 5.

The expression levels of chondrogenic and extracellular gene markers. Equal number of human chondrocytes were seeded in scaffold in presence of PRPr or FBS and monolayer seeded- cells were used as control. Cells were harvested after 72 hours and total RNA was extracted using Trizol method and the amount of 1 µg of pure RNA was used for gene expression analysis. Reverse-transcription was performed using cDNA synthesis kit to prepare first strand cDNA. Real time PCR was performed using SYBR Green PCR kit. scaffolds the expression level of COMP, Sox9, collagen type II, fibronectin and integrin β1 increased significantly when compared to controls. While, the differences in the expression levels of collagen types I, III and laminin were insignificant. The data were collected from three independent experiments. t-test * p < 0.05, ** p < 0.01