Fig. 5.

Differentiation capacity of MSCs derived from the human chorionic membranes. A Adipogenic differentiation stimulated cells stained with Oil Red O. Scale bar 100 μm. D show the magnification display of selected rectangular region within A. B Chondrogenic differentiation stimulated cells stained with Alcian blue. Scale bar 200 μm. E show the magnification display of selected rectangular region within B. C Osteogenic differentiation stimulated cells stained with von Kossa. Scale bar 200 μm. F show the magnification display of selected rectangular region within C. Expression of adipocyte-related genes FABP4, osteoblast-related genes Runx2 and chondrocyte-related genes Sox9 was detected by RT-PCR in induced hCMSCs and hCMSCs. GAPDH served as the internal control. MSCs: mesenchymal stem cells, hCMSCs: human chorionic membranes mesenchymal stem cells, RT-PCR: reverse transcription-polymerase chain reaction, FABP4: fatty acid binding protein 4, Runx2: Runt-related transcription factor 2