Table 1.

Enzyme digestion protocols used for DPSCs isolation

Enzymatic cocktail	Conditions	Teeth	References
3 mg/ml collagenase I, 4 mg/ml dispase	1 h at 37°C	Third molars, molars	[7]
3 mg/ml collagenase I, 4 mg/ml dispase	1-2 h at 37°C	Mouse incisor	[27]
4 mg/ml collagenase I, 4 mg/ml dispase II	24 h at 4°C	Deciduous teeth	[28]
4 mg/ml collagenase I, 2 mg/ml dispase	1 h at 37°C	Incisors	[29]
0.2 mg/ml collagenase I, 2 mg/ml dispase	70 min 37°C	Third molars	[30]
1 mg/ml collagenase I, 2.4 mg/ml dispase	1 h at 37°C	Deciduous teeth, adult molars	[31]
0.3 mg/ml collagenase I, 0.1% dispase II	1 h at 37°C	Deciduous teeth, premolars	[10]
1-3 mg/ml collagenase/dispase	20 min at 37°C	NS	[32]
1 mg/ml collagenase/dispase	30 min at 37°C	Third molars	[26]
3 mg/ml collagenase type I	1 h at 37°C	Third molars	[33]
3 mg/ml collagenase type I	40 min at 37°C	Third molars	[34]
0.2% collagenase type II	30 min at 37°C	Third molars	[35]
0.2% collagenase type I	1 h at 37°C	Incisors, canines, molars, deciduous teeth	[36]
1 mg/ml collagenase I	30 min at 37°C	Third molars	[18]
Accutase solution	30 min at 37°C	Third molars	[18]
0.04 mg/ml Liberase (mix collagenase I and II)	NS	Third molars	[37]
0.2% trypsin pretreated explants	5 min at 37°C	Third molars	[17]

NS not specified