Table 1.
The primer sequences used for RT-PCR
| Primer | Sequence | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|
| ALB |
F 5′ACAGAGACTCAAGTGTGCCAGT-3′
R 5′-GCAAGGTCCGCCCTGTCATC-3′ |
60 | 198 |
| CK-18 | 5′-AAATCCGGGAGCACTTGGAG-3′ 5′-CAATCTGCAGAACGATGCGG-3′ |
60 | 132 |
| CK-19 |
F 5′-ACTACACGACCATCCAGGAC-3′
R 5′-CCGTCTCAAACTTGGTTCGGA-3 |
60 | 126 |
| CYP2B6 |
F 5′-TTCTTCCGGGGATATGGTGT-3′
R 5′-TCCCGAAGTCCCTCATAGTG-3′ |
55 | 91 |
| G-6-P |
F 5′-CGACGAAGCGCAGACAG-3′
R 5′-GTATCCGACTGATGGAAGGC-3′ |
60 | 113 |
| HNF4 |
F 5′-AAGAAATGCTTCCGGGCTGG-3′
R 5′-GACGGGGGAGGTGATCTGTC-3′ |
60 | 156 |
| C met |
F 5′TAGGAAGAGGGCATTTTGGTTGTG-3′ R 5′-GCGAGAGGACATTGGGATGACTA-3′ |
60 | 174 |
| GAPDH |
F 5′-CTCTCTGCTCCTCCTGTTCG-3′
R 5′-ACGACCAAATCCGTTGACTC-3′ |
60 | 114 |
Italic letters indicate the sequences of primers