Fig. 2.

Differentiation capacities of MSCs populations toward osteoblast and adipocyte. A–D Spindle shaped fibroblastic-like cells in the primary culture of human MSCs derived from ICBM, Ad, and RIA. Human dermal fibroblast (HDF) is as control. E–H Osteogenic medium was used to MSCs and HDF cells induction at P2 toward osteoblasts for 21 days. MSCs after differentiation have produced calcified extracellular matrix that stained by Alizarin Red S reagent as evident for osteogenesis in comparison to HDF. I–L Additionally, osteogensis was confirmed by ALP assay of osteoblasts compared with HDF cells. M–P Also, Oil Red O staining was performed to staining of lipid droplets in order to prove adipogenic differentiation of MSCs, but not HDF, that were cultured in adipogenic induction medium. ICBM iliac crest bone marrow, Ad Adipose, RIA Reamer-irrigator-aspirator