Fig. 2.

Regenerating axons in 50 μm channels with addition of the SCs. A Freshly isolated DRG neurons are seeded and cultured for 5–6 days in the open area to allow the axons to grow and extend through the channels, whereupon SCs are subsequently seeded on the open area at the opposite end of the channels. B After 21 days of culture, Fluo-4 is added to permit fluorescent images of live cell staining with ×10 objective. Scale bar indicates 100 μm