Table 1.
Materials and company list
| Material | Company |
|---|---|
| Poly-l-lysine | Trevigen (Gaithersburg, MD) |
| Sodium bicarbonate | Sigma-Aldrich (St. Louis) |
| Poly-d-lysine | Sigma-Aldrich (St. Louis) |
| Fluoro-2 deoxy-uridine | Sigma-Aldrich (St. Louis) |
| Uridine | Sigma-Aldrich (St. Louis) |
| Cytosine β-d-arabinofuranoside (AraC) | Sigma-Aldrich (St. Louis) |
| Anti-Thy 1.1 antibody (cat. no. M-7898) | Sigma-Aldrich (St. Louis) |
| Rabbit complement | Sigma-Aldrich (St. Louis). |
| Heat inactivated fetal bovine serum | Hyclone (Logan, Utah) |
| Bovine pituitary extract (BPE) | Clonetics (Allendale, NJ) |
| Forskolin | Calbiochem (Billerica, MA) |
| Type I collagenase | Worthington (Lakewood, NJ) |
| Neurobasal medium 1× | Invitrogen (Carlsbad, CA) |
| B-27 supplement | Invitrogen (Carlsbad, CA) |
| Glutamax-I | Invitrogen (Carlsbad, CA) |
| Albumax-I | Invitrogen (Carlsbad, CA) |
| Nerve growth factor | Invitrogen (Carlsbad, CA) |
| Dulbecco’s modified Eagle medium (DMEM) | Invitrogen (Carlsbad, CA) |
| Penicillin and streptomycin | Invitrogen (Carlsbad, CA) |
| 0.25% trypsin–EDTA | Invitrogen (Carlsbad, CA) |
| 1× phosphate buffered saline (PBS) | Invitrogen (Carlsbad, CA) |
| HEPES buffer | Invitrogen (Carlsbad, CA) |
| Fluo-4 AM | ThermoFisher Scientific (Grand Island, NY) |
| Cell permeant (cat. no. F-14201) | ThermoFisher Scientific (Grand Island, NY) |
| Bovine serum albumin (BSA) | US Biological (Marblehead, MA) |
| Rabbit anti-myelin protein zero antibody (cat. no. ab31851) | Abcam (Cambridge, MA) |
| Mylar films | InfinityGraphics (East Lansing, MI) |
| Type I collagen (cat. no. #354236) | Corning (Corning, NY) |