Fig. 4.

Analysis of the miR-214 binding to the EZH2 3′-UTR by the luciferase activity assay. A miR-214 sequence with the 3′-UTRs of EZH2 in different species. The position of the miR-214 binding site on human EZH2 mRNA and the EZH2 3′-UTR mutant is depicted. B Addition of miR-214 mimic attenuated the EZH2 3′-UTR reporter gene activity. Mutation of the miR-214 putative target site blocked the suppressive effect of miR-214 on the target, suggesting EZH2 as a miR-214 target gene. p < 0.05 versus control mimic. C TOP Flash and FOP Flash assays assessed the effect of miR-214 and EZH2 interference on the Wnt/β-catenin signaling pathway (*p < 0.05, **p < 0.01)