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Alizarin Red staining on 21th day of UCSCs’ osteogenesis (calcium deposits formed in the differentiated cells (osteoblasts) are orange/red color). A Positive control (differentiated cells without HCP). B Negative control (cells cultured in culture medium without ostegenesis factors). C HCP 10−5 M, D HCP 10−6 M, E HCP 10−7 M. Images acquired by inverted light microscope (ZEISS AX10, Poland; magnification ×100)
