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. 2018 Jan 8;15(2):211–222. doi: 10.1007/s13770-017-0103-9

Fig. 9.

Fig. 9

Alizarin Red staining on 21th day of ADSCs osteogenesis (calcium deposits formed in the differentiated cells (osteoblasts) are red–orange/red color). A Positive control (differentiated cells without preincubation with HCP in osteogenic induction medium). B Negative control (cells cultured in culture medium without preincubation with HCP). C–E Differentiated cells preincubated with HCP before the beginning of the differentiation process. C 10−4 M HCP. D 10−5 M HCP. E 10−6 M HCP. F 10−7 M HCP. Images acquired by inverted light microscope (ZEISS AX10, Poland; magnification ×100)