Fig. 3.

Colony formation ability of hFCPCs. Colony formation ability of hFCPCs from donor 2 was examined by the CFU-F assay. A hFCPCs cultured in α-MEM with 10% FBS or SFM-XF were plated at 10 or 230 cells per 35 mm dish and subjected to the CFU-F assay at passage 2 for 14 days. Unexpectedly, there was no colony obtained in SFM-XF medium. B hFCPCs cultured in α-MEM with 10% FBS or SFM-XF were both suspended in α-MEM with 10% FBS and plated at 10 cells per 35 mm dish at passage 5 for the CFU-F assay. The colony forming efficiency and the diameter of the colonies were represented as the mean value with S.D. from three independent experiments (n = 3). C The CFU-F assay was performed with hFCPCs cultured in α-MEM with 10% FBS at passages 2, 5, 10, and 20. The colony forming efficiency and diameter of colonies were measured as above and presented in the graphs (n = 3). *p < 0.05; **p < 0.01