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. 2018 Jun 13;99(8):1012–1026. doi: 10.1099/jgv.0.001083

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© 2018 Reproduced with the permission of Public Health England under delegated authority from the Controller of HMSO

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — Performance of the ZIKV RT-RPA assay on a portable battery-operated instrument, the Genie III by OptiGene. (a) Tenfold dilution series of Zika synthetic RNA template 5 (KU365780) used to determine the lowest number of target molecules detected by the ZIKV RT-RPA assay. The insert displays the fluorescent signal generated from the amplification of 500, 50 and 5 copies of ZIKV synthetic RNA template 5 in comparison to the non-template control (NTC) in the ZIKV RT-RPA assay. (b) Tenfold dilution series of extracted nucleic acid from two strains of cultured ZIKV (African – KY288905, left panel, and South American – KU501215, right panel), as detected by the ZIKV RT-RPA assay and compared to the non-template control (NTC). (c) Detection of clinical samples from two patients in duplicates with low ZIKV titre (patient 1) and high ZIKV titre (patient 2) in comparison to 5×10³ and 5×10² copies of Zika synthetic RNA template 5 (KU365780) and the non-template control (NTC) by the ZIKV RT-RPA.