Table 2. Time to positive (TTP) signal in the detection of different amounts of ZIKV synthetic RNA template 5 (from 5×106 to 5×102 copies per reaction) using the ZIKV RT-RPA assay.
For comparison, the time necessary to detect the target using the published Zika RT-PCR is also shown.
| Template 5 (KU365780) copies/rxn | RT-RPA TTP (min) | RT-RPA result | RT-PCR TTP (min) | RT-PCR result |
|---|---|---|---|---|
| 5×106 | 3.38 | + (5/5) | 40.12 | + (3/3) |
| 5×105 | 3.84 | + (5/5) | 45.07 | + (3/3) |
| 5×104 | 4.76 | + (5/5) | 49.18 | + (3/3) |
| 5×103 | 6.48 | + (5/5) | 53.38 | + (3/3) |
| 5×102 | 10.72 | + (5/5) | 57.25 | + (3/3) |
| NTC | Not detected | − (5/5) | Not detected | − (3/3) |