Table 3. Time to positive (TTP) signal in the detection different strains of ZIKV, based on both synthetic RNA fragments (1–5) and extracted nucleic acid from cultured virus of two Zika virus strains (African – KY288905 and South American – KU501215); the ZIKV RT-RPA assay is compared to the performance of the published ZIKV RT-PCR assay.
| Sequence ID | Description | RT-RPA TTP (min) | RT-RPA result | RT-PCR TTP (min) | RT-PCR result |
|---|---|---|---|---|---|
| Template 1 (EU545988) | Synthetic RNA | 8.24 | + (3/3) | 53.75 | + |
| Template 2 (KJ776791) | Synthetic RNA | 7.72 | + (3/3) | 54.05 | + |
| Template 3 (KU321639) | Synthetic RNA | 7.89 | + (3/3) | 54.24 | + |
| Template 4 (KU365778) | Synthetic RNA | 8.13 | + (3/3) | 54.65 | + |
| Template 5 (KU365780) | Synthetic RNA | 7.32 | + (3/3) | 53.39 | + |
| African Zika virus (KY288905) | Extracted virus | 7.53 | + (3/3) | 49.16 | + |
| South American Zika virus (KU501215) | Extracted virus | 4.52 | + (3/3) | 45.95 | + |