Table 5. Detection of ZIKV nucleic acid using the ZIKV RT-RPA assay in a selection of clinical samples.

The TTP signal obtained from the ZIKV RT-RPA assay is indicated for a range of patient samples, including semen and urine, and is compared to that generated by the published ZIKV RT-PCR.

Sample No	Sample description	RT-RPA TTP (min)	RT-RPA result	RT-PCR result
1	Other clinical	13.9	+	+
2	Other clinical	34.8	+	+
3	Other clinical	6.3	+	+
4	Other clinical	15.5	+	+
5	Other clinical	7.2	+	+
6	Semen	37.5	+	+
7	Semen	16.9	+	+
8	Semen	18.2	+	+
9	Semen	Not detected	−	+
10	Semen	6.4	+	+
11	Semen	Not detected	−	+
12	Semen	5.2	+	+
13	Semen	Not detected	−	−
14	Serum	24.8	+	+
15	Serum	37.5	+	+
16	Serum	Not detected	−	−
17	Serum	Not detected	−	−
18	Serum	Not detected	−	−
19	Urine	Not detected	−	+
20	Urine	Not detected	−	−
21	Urine	19.3	+	+
22	Urine	22.5	+	+
23	Urine	34.6	+	+
24	Urine	Not detected	−	−
25	Urine	Not detected	−	−
26	Urine	15.3	+	+
27	Urine	16.2	+	+
28	Urine	Not detected	−	+
29	Urine	33.1	+	+
30	Urine	Not detected	−	−
31	Urine	Not detected	−	−
32	Urine	21.4	+	+
33	Urine	37.5	+	+
34	Urine	35.2	+	+
35	Urine	22.5	+	+
36	Urine	Not detected	−	−
37	Urine	Not detected	−	−
38	Urine	29.5	+	+
39	Urine	36.5	+	+
40	Urine	Not detected	−	−
41	Urine	28.7	+	+
42	Urine	28.8	+	+
43	Urine	Not detected	−	+
44	Urine	Not detected	−	+
45	Urine	Not detected	−	−
46	Urine	24.5	+	+
47	Urine	Not detected	−	−
48	Urine	27.5	+	+
49	Urine	16.3	+	+
50	Urine	Not detected	−	−
51	Urine	Not detected	−	+
52	Urine	19.6	+	+
53	Urine	8.1	+	+
54	Whole blood	20.8	+	+
55	Whole blood	29.0	+	+