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. 2018 Jun 13;99(8):1012–1026. doi: 10.1099/jgv.0.001083

Table 5. Detection of ZIKV nucleic acid using the ZIKV RT-RPA assay in a selection of clinical samples.

The TTP signal obtained from the ZIKV RT-RPA assay is indicated for a range of patient samples, including semen and urine, and is compared to that generated by the published ZIKV RT-PCR.

Sample No Sample description RT-RPA TTP (min) RT-RPA result RT-PCR result
1 Other clinical 13.9 + +
2 Other clinical 34.8 + +
3 Other clinical 6.3 + +
4 Other clinical 15.5 + +
5 Other clinical 7.2 + +
6 Semen 37.5 + +
7 Semen 16.9 + +
8 Semen 18.2 + +
9 Semen Not detected +
10 Semen 6.4 + +
11 Semen Not detected +
12 Semen 5.2 + +
13 Semen Not detected
14 Serum 24.8 + +
15 Serum 37.5 + +
16 Serum Not detected
17 Serum Not detected
18 Serum Not detected
19 Urine Not detected +
20 Urine Not detected
21 Urine 19.3 + +
22 Urine 22.5 + +
23 Urine 34.6 + +
24 Urine Not detected
25 Urine Not detected
26 Urine 15.3 + +
27 Urine 16.2 + +
28 Urine Not detected +
29 Urine 33.1 + +
30 Urine Not detected
31 Urine Not detected
32 Urine 21.4 + +
33 Urine 37.5 + +
34 Urine 35.2 + +
35 Urine 22.5 + +
36 Urine Not detected
37 Urine Not detected
38 Urine 29.5 + +
39 Urine 36.5 + +
40 Urine Not detected
41 Urine 28.7 + +
42 Urine 28.8 + +
43 Urine Not detected +
44 Urine Not detected +
45 Urine Not detected
46 Urine 24.5 + +
47 Urine Not detected
48 Urine 27.5 + +
49 Urine 16.3 + +
50 Urine Not detected
51 Urine Not detected +
52 Urine 19.6 + +
53 Urine 8.1 + +
54 Whole blood 20.8 + +
55 Whole blood 29.0 + +