(A) EMT-related gene (E-cadherin, Vimentin, Fibronectin, TWIST1, and SOX2) expression in parental, si-control and si-NOTCH4 cells is measured by qRT-PCR. The expression differences between si-control and si-NOTCH4 cells are compared. P value is calculated by using Student’s t-test. *: P < 0.05, **: P < 0.01, N.S.: not significant. (B) Western blots of EMT-related genes in si-control and si-NOTCH4 of SKN3 and Cal27 cells. GAPDH antibody is used as a control.