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. 2018 Oct;153:86–98. doi: 10.1016/j.biochi.2018.06.020

Fig. 3.

Fig. 3

LC-HRMS RICs (585.4059 ± 10 ppm) corresponding to [M]+ ions of [2H5]GP derivatised dihydroxy-3-oxocholest-4-en-26-oic acids. (A) Control CSF (upper panel), authentic standard of (25 R)CA4-7α,24 S-diol-3-one (lower panel). (B) Control CSF (upper panel), authentic standard of (25 R/S)CA4-7α,25-diol-3-one (lower panel). (C) Control CSF (SPE1-Fr1A) treated with cholesterol oxidase followed by [2H5]GP reagent (upper panel), control CSF (SPE1-Fr1B) treated with [2H0]GP reagent in the absence of cholesterol oxidase (lower panel). In (C) the RICs for [2H5]GP derivatised and [2H0]GP derivatised acids are plotted with the same y-axis. Data was acquired in the Orbitrap analyser of the Orbitrap Elite instrument. Peaks corresponding to the dihydroxy-3-oxocholest-4-en-26-oic acids CA4-7α,25-diol-3-one, CA4-7α,12α-diol-3-one, CA4-7α,24-diol-3-one and CA4-7α,x-diol-3-one are colour coded green, gold, red and purple respectively.