Fig. 8.

LC-HRMS RICs ±10 ppm appropriate to the analysis of [²H₅]GP and [²H₀]GP derivatised dihydroxy-3-oxocholest-4-en-26-oic acids in control (A), CTX (C) and SPG5 (D) plasma. The RICs for [²H₅]GP derivatised dihydroxy-3-oxocholest-4-en-26-oic acids are plotted on the same y-axis scale as the RICs for [²H₀]GP derivatised acids. (B) LC-MS³ TICs appropriate to [²H₅]GP and [²H₀]GP derivatised dihydroxy-3-oxocholest-4-en-26-oic acids in control plasma. Control, CTX and SPG5 plasma were analysed on different days which causes a small deviation in retention time. Peaks were correlated by recording QCs with each sample batch. HRMS data was recorded in the Orbitrap and MS³ data in the LIT of the Orbitrap Elite hybrid instrument. Peaks are colour coded as in Fig. 3.