Table 1.

Concentrations of dihydroxy-3-oxocholest-4-en-26-oic acids in CSF.

Compound	Control CSF, n = 42			CTX CSF, n = 6				SPG5 CSF, n = 3
	Mean	Median	SD	Mean	Median	SD	Significance1	Mean	Median	SD	Significance1
	ng/mL	ng/mL	ng/mL	ng/mL	ng/mL	ng/mL	CTX v Control	ng/mL	ng/mL	ng/mL	SPG5 v Control
CA4-7α,24-diol-3-one	0.30	0.30	0.08	0.10	0.10	0.02	P < 0.01	0.07	0.09	0.07	P < 0.01
CA4-7α,x-diol-3-one	5.83	5.65	1.49	ND	ND	NA	P < 0.01	0.60	0.27	0.66	P < 0.01
CA4-7α,25-diol-3-one	1.60	1.51	0.44	ND	ND	NA	P < 0.01	0.192	0.05	0.27	P < 0.01
(25 R)CA4-7α,12α-diol-3-one	1.11	0.82	1.10	0.49	0.29	0.60	P < 0.05	0.64	0.72	0.17	P > 0.05
(25 S)CA4-7α,12α-diol-3-one	ND	ND	NA	0.12	0.09	0.11	P < 0.01	ND	ND	NA	NA

Quantification was made utilising LC-HRMS RICs with [²H₇]22 R-hydroxycholest-4-en-3-one as the internal standard.

1. Mann-Whitney test.

2. From Ref. [9].

ND = not detected.

NA = not available.