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. 2018 Sep 6;3(17):e120824. doi: 10.1172/jci.insight.120824

Figure 6. Lipid analysis of Abca1/g1–m/–m mice eyes at 12 months of age demonstrates focal subretinal and RPE deposits with increased cholesterol metabolites.

Figure 6

(A) Representative Oil Red O staining of 12-month-old Abca1/g1F/F and Abca1/g1–m/–m mice retinas. Note the lipid deposits within the RPE (yellow arrowheads) or subretinal space (red arrowhead). Scale bar: 20μm. (B) Analyses of free cholesterol, 3β,5α,6β-cholestanetriol (C-triol), 4β-hydroxycholesterol (4β-HC), 24-hydroxycholesterol (24-HC), 25-HC, 27-HC, and 7-ketocholesterol in retina/RPE/choroid complex at 12 months of age (normalized peak area ratio/mg wet tissue weight). Abca1/g1F/F, n = 7; Abca1/g1–m/–m, n = 6. *P < 0.05 and **P < 0.01 by 2-tailed unpaired t test. (C) Analyses of cholesterol esters (CE) in retina/RPE/choroid complex at 12 months of age (normalized peak area ratio/mg wet tissue weight). Abca1/g1F/F, n = 7; Abca1/g1–m/–m, n = 6. **P < 0.01 and ***P < 0.001 by 2-tailed unpaired t test. Values are mean ± SEM.