Figure 2. Mutational landscape of metastatic HPV-associated oropharyngeal cancer.

(A) Normalized total mutational burden (TMB) including all nonsynonymous gene alterations per sample (n = 39). (B) Mutational plot showing the most frequently mutated genes (top to bottom, >5% frequency) with gene frequency listed at right (%) among 42 metastatic HPV⁺ oropharyngeal tumors. The vertical bar graph shows mutational frequency compared with whole-exome sequencing results among n = 72 patients in the The Cancer Genome Atlas–Pan Cancer Atlas (TCGA-PCA) and n = 51 in the University of Chicago cohorts with nonmetastatic HPV-associated head and neck cancer. Gene frequency (%) is also shown among both comparator cohorts. Significance evaluated by Bonferroni-corrected 2-sided χ² test.