Figure 3. Mutational landscape in metastatic HPV-associated oropharyngeal cancer by site of disease.

(A) Mutational plot showing the most frequently altered genes (top to bottom) among metastatic HPV⁺ tumors with only pulmonary metastases (n = 20), and in those with (B) extrapulmonary sites of metastatic disease (n = 22). Normalized total mutational burden (TMB) is shown in the bar graph above. Smoking status is depicted by colored shading in the bar graph: blue represents never smokers, dark gray represents former (>10 pack-years) or current smokers. Each column represents an individual patient sample and they are arranged according to where the biopsy material for massively parallel sequencing was obtained (primary site of disease or metastatic focus).