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. 2018 Sep 6;3(17):e91954. doi: 10.1172/jci.insight.91954

Figure 2. Kinetics of changes in the expression of activation markers following stimulation of cultured human iNKT cells.

Figure 2

(A) Images of cultured human iNKT cells after 29 hours of incubation without stimulation or during stimulation with αCD3 and αCD28 Abs as described in the Methods. Scale bars: 250 μm. (B and C) FACS analysis reveals reduced immunofluorescence of CD3 and the invariant TCR (Vα24-Jα18) over time following iNKT cell stimulation by αCD3/αCD28 (Stim) and lack of change in response to IL-12 and IL-18. (D) Kinetics of changes in CD69, T-bet, and adenosine A2A receptor (A2AR) expression over time in response to αCD3/αCD28 or IL-12/IL-18 normalized to the maximal response of each marker. (E) Absolute changes in phospho-NF-κB p65, CD69, T-bet, and A2AR immunofluorescence over time in response to αCD3/αCD28 (TCR) or IL-12/IL-18 (cytokines).