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. 2018 Sep 6;3(17):e120196. doi: 10.1172/jci.insight.120196

Figure 2. Deficiency of lipocalin-2 protects mice from ANS-induced glomerular injuries in the kidney.

Figure 2

(A) WT, Adipo-LKO, and LKO mice were subjected to sham or 4-week ANS challenge as in Figure 1. At the end of treatment, urine samples were collected for measuring albumin levels as described in Methods. (B) The mRNA expression of wt1 in kidney was evaluated by qPCR and presented as fold changes against WT sham controls. (C) Morphology of the glomeruli was examined in kidney tissue sections by both H&E (upper row) and PAS (lower row) staining. Magnification, 400×. Scale bar: 20 μm. (D) The glomerular area was quantified using ImageJ software and expressed as glomerular volume (D, left). The protein levels of synaptopodin were determined by Western blotting using the tissue lysates prepared from kidney samples (D, right). Data are shown as mean ± SEM; *P < 0.05 vs. corresponding sham controls; #P < 0.05 vs. WT ANS by Mann-Whitney nonparametric Student’s t test (n = 6–8). EPL, eplerenone.