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(A) Chromosome 17q12 genomic locus/HER2 amplicon. Genomic location and orientation of MIEN1 gene (M) in relation to HER2 and GRB7 within the HER2 amplicon. (B) Structure of MIEN1 gene. Exons of MIEN1 with locations of the redox, immunoreceptor tyrosine activation motif (ITAM) and prenylation domains. Location of sgRNA sequences are denoted by vertical black lines.
