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. 2018 Oct 4;13(10):e0204869. doi: 10.1371/journal.pone.0204869

Table 1. Feed components and proximate composition of the experimental diets on an as-fed basis.

D1 D2 D3 D4
Ingredients 1st 2nd 1st 2nd 1st 2nd 1st 2nd
Extruded linseed % 0.00 0.00 5.00 5.00 5.00 5.00 5.00 5.00
Barley meal % 85.50 91.00 80.50 86.60 80.30 86.40 80.50 86.60
Soya bean meal % 11.00 5.50 11.00 5.00 11.00 5.00 11.00 5.00
L-Lysine % 0.31 0.29 0.30 0.29 0.30 0.29 0.30 0.29
DL-Methionine % 0.06 0.04 0.06 0.03 0.06 0.03 0.06 0.03
L-Threonine % 0.05 0.04 0.05 0.03 0.05 0.03 0.05 0.03
Calcium carbonate % 1.18 1.13 1.19 1.15 0.89 0.85 1.19 1.15
Dicalcium phosphate % 1.00 1.10 1.00 1.00 1.00 1.00 1.00 1.00
Salt (NaCl) % 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40
Vitamin/mineral pre-mix1 % 0.50 0.50 0.50 0.50 0.50 0.50 0.50 0.50
Vitamin E and Selenium pre-mix2 % 0.00 0.00 0.00 0.00 0.50 0.50 0.00 0.00
Plant extracts (Grape-skin + oregano) g per kg of feed 0.00 0.00 0.00 0.00 0.00 0.00 3.00+2.00 3.00+2.00
Proximate composition
Digestible energy kcal/kg 3189 3168 3255 3235 3248 3228 3255 3235
Crude protein % 14.89 11.31 15.39 11.73 15.37 11.71 15.39 11.73
Crude fat % 1.75 1.74 3.58 3.58 3.58 3.58 3.58 3.58
Crude fiber % 4.33 4.2 4.62 4.48 4.61 4.47 4.62 4.48
Ca % 0.80 0.79 0.82 0.79 0.82 0.79 0.82 0.79
P % 0.54 0.54 0.55 0.53 0.55 0.53 0.55 0.53
Fatty acid composition % (of total fatty acids)
C14:0 % 0.47 0.39 0.25 0.21 0.25 0.22 0.26 0.22
C16:0 % 29.01 24.25 18.13 15.20 17.78 15.59 18.80 15.31
C16:1 % 0.49 0.34 0.17 0.15 0.17 0.17 0.02 0.15
C18:0 % 2.03 1.51 4.00 3.18 3.88 3.34 4.16 3.23
C18:1 n-9 % 14.92 13.50 20.60 18.12 20.24 18.45 21.29 18.26
C18:2 n-6 % 47.55 53.67 33.50 34.69 33.91 34.09 32.52 34.47
C18:3 n-3 % 4.77 5.70 22.83 28.02 23.25 27.73 22.38 27.95
C20:1 % 0.74 0.64 0.53 0.41 0.52 0.42 0.57 0.41

D1 = standard diet for growing-finishing pigs; D2 = standard diet supplemented with linseed (source of n-3 PUFA); D3 = standard diet supplemented with linseed, vitamin E and selenium (250 and 0.31 mg per kg of feed respectively; D4 = standard diet supplemented with linseed and plant extracts from grape-skin (3 g per kg of feed) and oregano (2 g per kg of feed) as polyphenols source (total polyphenols added 37.6 mg per kg of feed).

1st = feed administered from an average weight of 79.9 kg to 113.4 kg; 2nd = feed administered from an average weight of 113.4 kg to slaughter.

1Provided the following nutrients (per kg diet as-fed): Vitamin A 15,000 IU; Vitamin D3 2,000 IU; Vitamin E alpha-tocopheryl-acetate), 50 mg; Vitamin K, 2.5 mg; Vitamin B1, 2 mg; Vitamin B2 5 mg; Vitamin B5, 15 mg; Vitamin B6, 4 mg; Vitamin B12, 0.036 mg; Niacin, 25 mg; Folic acid, 1 mg; Biotin, 0.15 mg; Choline, 346 mg; Cu, 15 mg; Fe, 150 mg; Mn, 25 mg; Co, 0.4 mg; I, 1.5 mg; Zn, 100 mg; Se) 0.1 mg.

2Provided the following nutrients (per kg diet as-fed): Vitamin E (alpha-tocopheryl-acetate), 200 mg and Se, 0.21 mg.