Skip to main content
. 2018 Sep 24;14(9):e1007689. doi: 10.1371/journal.pgen.1007689

Fig 10. Bacterial two hybrid assay.

Fig 10

pT18-containing glmR and the compatible plasmid pT25 containing glmS or cdaA were transformed into E. coli strain BTH101. When co-expressed protein fusions interact, the Bordetella pertussis adenylate cyclase is active as assessed by blue color in the presence of X-gal. Negative controls include cells containing one or both empty vectors, and the positive control is the pT18-ZIP and pT25-ZIP plasmids [60]. Left panel is a single plate, and the right panel is images from a separate experiment showing a lack of signal for interaction of GlmR with CdaR or GlmM and the corresponding positive control.