Figure 4. Involvement of NOS, COX, or EDH restoration of the acetylcholine-induced reduction in blood pressure. Twenty-one days after ligature or sham procedure, the animals were prepared for measurement of mean arterial pressure, and dose response curves to acetylcholine and sodium nitroprusside were performed before and 30 min after L-NAME (20 mg/kg, i.v; panels A-B), indomethacin (10 mg/kg, i.v; panels C-D) or apamin plus TRAM-34 (75 μg/kg and 1 mg/kg, respectively, i.v; panels E-F). The change in mean arterial pressure (% of decrease from basal blood pressure) was determined. Statistical analyses were performed using two-way analysis of variance followed by Bonferroni post hoc test. *p<0.05.