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. 2018 Oct 2;26:e20170566. doi: 10.1590/1678-7757-2017-0566

Figure 4. Building the E. faecalis LuxS deficient strain ΔLuxS 29212 by LFH-PCR.

Figure 4

Amplification of the upper and down-stream homologous arm of the E. faecalis LuxS gene. M: Marker DL2000 1: LuxS upstream homologous arm fragment (P1 to P2), length is 961 bp; 2: LuxS down-stream homologous arm fragment (P3 to P4), length is 820 bp; (b) The identification of recombinant plasmid. M: Marker 1: Recombinant plasmid 2: pEASY-T1 vector; (c) The identification of the LuxS gene knockout in E. faecalis . M: DNA markerDGL 4000; Lane 1-5, upper band: recombination vector LuxS up and down homologous arm fragment interpose with the amp and kana genes; lower band: LuxS gene from up to low homologous arm (P1 to P4)